Advanced Glycation End Products in Children With Type 1 Diabetes: Family Matters?

A dvanced glycation end product (AGE) burden can be indirectly quantified in vivo by measuring skin autofluorescence (SAF) (1). It has been recently published by Felipe et al. (2) that SAF is correlated with glycated he-moglobin (GHb) but not with mean blood glucose (MBG) in type 1 diabetic children. This suggests that factors besides MBG exposure may be influential in skin AGE generation. In the current study, we have compared SAF in type 1 diabetic children and their nondiabetic sibling. We included 52 type 1 diabetic children, 55% boys, aged 12 (6) years [median (interquartile range: 75th per-centile 2 25th percentile)], and 28 non-diabetic siblings, 46% boys, aged 11 (6) years. Among diabetic children, the duration of diabetes was 71 (45) months (min 13 to max 135) and median GHb levels since diabetes discovery were 8.0 (1.1) %. Diabetic children and sibling control groups were balanced in terms of BMI, waist circumference (WC), and blood pressure (BP). Informed consent was obtained from the children and their parents. At the time of the clinic visit between 2 and 6 P.M., SAF measurements were performed using the AGE Reader (DiagnOptics, Groningen, the Nether-lands). Three consecutive SAF measurements per subject were carried out in the forearm of participants. SAF was expressed in arbitrary units (AUs) as the mean of the three separate measures. Comparisons were made using the Mann-Whitney test. Factors potentially associated with SAF levels were studied using a multivariate regression model with SAF level as the dependent variable and age and sex as confounding factors (2,3). Diabetic children had significantly elevated SAF levels compared with the sibling control group (1.36 [0.32] vs. 1.20 [0.24] AU, P , 0.001). In diabetic patients, SAF levels were associated with last GHb levels when adjusted for age and sex (r 5 0.33, P , 0.05). No significant associations were observed between SAF levels and BMI, WC, BP, duration of diabetes, capillary blood glucose, and median GHb. However, a significant correlation of SAF levels was observed among sib-lings (binomial diabetic – nondiabetic siblings), even when adjusted for diabetic GHb and age (n 5 27, r 5 0.43, P 5 0.01). To our knowledge, our data are the first to report an increased SAF levels early in the medical history of diabetic children compared with sibling control subjects. The correlation between SAF and recent but not median GHb possibly reflects middle-term interactions with hyperglycemia. The association observed …